[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aim and Scope
Content Coverage
Ethics
Conflict of Interest
Copyrights & License
Price
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Current Issue
All Issues
Instruction to Authors::
Peer-Review::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 18, Issue 1 (3-2016) ::
J Gorgan Univ Med Sci 2016, 18(1): 45-52 Back to browse issues page
Evaluation of anti-microbial activity of Lactobacillus acidophillus and Lactobacillus ruteri against entero-pathoges by in vitro and in vivo methods
Soltan Dallal MM * 1, Keshtvarz M2 , Zamani S3 , Shirazi L4
1- Professor, Div. of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , msoltandallal@gmail.com
2- Ph.D Candidate in Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3- Ph.D Candidate in Microbiology, School of Medicine, Tehran University of Medical Sciences. Tehran, Iran
4- M.Sc in Microbiology, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (10376 Views)

Background and Objective: Probiotics are beneficial organisms therapeutic within microbial flora. Shigella, Escherichia coli and Salmonella are the most common cause of intestinal infectious diseases that lead to morbidity and mortality in infant and children worldwide. The aim of this study was to evaluate anti-microbial activity of Lactobacillus acidophillus and Lactobacillus ruteri against entero-pathoges by in vitro and in vivo methods. Methods: In this experimental study, the therapeutic effect of the lactobacillus acidophilus ATCC 4356 and ruteri ATCC 23272 against Shigella sonnei ATCC 9290, Escherichia coli ATCC 25922 and Salmonella enterica BAA-708 were evaluated by in vitro (spot agar) and in vivo (BALB/c mice) methods. Weight improvment and survival rate in mice were recorded. Results: Lactobacillus acidophillus and ruteri had protective and therapeutic effect against diarrhea caused by pathogenic bacteria. Probiotics reduced the weight, colonization of pathogens and increased the survival rate of animals (P<0.05). Conclusion: Lactobacillus acidophillus and ruteri has anti-microbial activity and their consumption can be effective in the prevention and also the treatment of intestinal disease.
Keywords: Entero pathogens, Probiotics, Antibacterial activity, Mouse
Full-Text [PDF 272 kb] [English Abstract]   (17730 Downloads) |   |   Abstract (HTML)  (261 Views)  
Type of Study: Original Articles | Subject: Microbiology
References
1. You D, Jones G, Wardlaw T. Levels and Trends in Child Mortality Report 2011: Estimaes developed by the UN Inter-agency Group for Child Mortality Estimation. 35th. New York: UNCF. 2011; pp: 1-24.
2. Huilan S, Zhen LG, Mathan MM, Mathew MM, Olarte J, Espejo R, et al. Etiology of acute diarrhoea among children in developing countries: a multicentre study in five countries. Bull World Health Organ. 1991; 69(5):549-55.
3. World Health Organization, UNICEF/WHO, Diarrhoea: Why children are still dying and what can be done, 2009. Available at: http://www.who.int/maternal_child_adolescent/documents/cah_adh_flyer_2010_12_en.pdf
4. Meng CY, Smith BL, Bodhidatta L, Richard SA, Vansith K, Thy B, et al. Etiology of diarrhea in young children and patterns of antibiotic resistance in Cambodia. Pediatr Infect Dis J. 2011 Apr; 30(4):331-5. doi:10.1097/INF.0b013e3181fb6f82
5. Das JK, Mishra D, Ray P, Tripathy P, Beuria TK, Singh N, Suar M. In vitro evaluation of anti-infective activity of a Lactobacillus plantarum strain against Salmonella enterica serovar Enteritidis. Gut Pathog. 2013 May; 5(1):11. doi:10.1186/1757-4749-5-11
6. McFarland LV. Meta-analysis of probiotics for the prevention of traveler's diarrhea. Travel Med Infect Dis. 2007 Mar;5(2):97-105.
7. Cross ML, Mortensen RR, Kudsk J, Gill HS. Dietary intake of Lactobacillus rhamnosus HNOO1 enhances production of both Th1 and Th2 cytokines in antigen-primed mice. Med Microbiol Immunol. 2002 May;191(1):49-53.
8. Puertollano E, Puertollano MA, Cruz-Chamorro L, Alvarez de Cienfuegos G, Ruiz-Bravo A, de Pablo MA. Orally administered Lactobacillus plantarum reduces pro-inflammatory interleukin secretion in sera from Listeria monocytogenes infected mice. Br J Nutr. 2008 Apr;99(4):819-25.
9. Jiang T, Savaiano DA. Modification of colonic fermentation by bifidobacteria and pH in vitro. Impact on lactose metabolism, short-chain fatty acid, and lactate production. Dig Dis Sci. 1997 Nov; 42(11):2370-7.
10. Salminen S, Collado MC, Isolauri E, Gueimonde M. Microbial-host interactions: selecting the right probiotics and prebiotics for infants. Nestle Nutr Workshop Ser Pediatr Program. 2009; 64:201-13. doi:10.1159/000235792
11. Nomoto K. Prevention of infections by probiotics. J Biosci Bioeng. 2005 Dec;100(6):583-92.
12. Rolfe RD. The role of probiotic cultures in the control of gastrointestinal health. J Nutr. 2000 Feb; 130(2S Suppl): 396S-402S.
13. Kailasapathy K, Chin J. Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp. Immunol Cell Biol. 2000;78:80-8. doi:10.1046/j.1440-1711.2000.00886.x
14. Axelsson LT, Chung TC, Dobrogosz WJ, Lindgren SE. Production of a broad spectrum antimicrobial substance by Lactobacills reuteri. Microb Ecol Health Dis. 1989; 2(2):131-36.
15. Chung TC, Axelsson L, Lindgren E, Dobrogosz WJ. In vitro studies on reuterin synthesis by Lactobacillus reuteri. Microb Ecol Health Dis. 1989 ;( 2): 137-44.
16. Tabasco R, García-Cayuela T, Peláez C, Requena T. Lactobacillus acidophilus La-5 increases lactacin B production when it senses live target bacteria. Int J Food Microbiol. 2009 Jun; 132(2-3):109-16. doi:10.1016/j.ijfoodmicro.2009.04.004
17. Erdogrul O, Erbilir F. Isolation and Characterization of Lactobacillus bulgaricusand Lactobacillus caseifrom Various Foods. Turk J Biol. 2006; 30:39-44.
18. Cano PG, Perdigón G. Probiotics induce resistance to enteropathogens in a re-nourished mouse model. J Dairy Res. 2003 Nov; 70(4):433-40.
19. de LeBlanc Ade M, Castillo NA, Perdigon G. Anti-infective mechanisms induced by a probiotic Lactobacillus strain against Salmonella enterica serovar Typhimurium infection. Int J Food Microbiol. 2010 Apr; 138(3):223-31. doi:10.1016/j.ijfoodmicro.2010.01.020
20. Onunbawo ST, Sanni AI, Onilude AA. Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis. Afr J Biotechnol. 2003; 2(8); 219-27.
21. Coconnier MH, Lievin V, Hemery E, Servin AL. Antagonistic activity against Helicobacter infection in vitro and in vivo by the human Lactobacillus acidophilus strain LB. Appl Environ Microbiol. 1998 Nov;64(11):4573-80.
22. de LeBlanc Ade M, Castillo NA, Perdigon G. Anti-infective mechanisms induced by a probiotic Lactobacillus strain against Salmonella enterica serovar Typhimurium infection. Int J Food Microbiol. 2010 Apr; 138(3):223-31. doi:10.1016/j.ijfoodmicro.2010.01.020
23. Spinler JK, Taweechotipatr M, Rognerud CL, Ou CN, Tumwasorn S, Versalovic J. Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe. 2008 Jun;14(3):166-71. doi:10.1016/j.anaerobe.2008.02.001
24. Kolader ME, Vinh H, Ngoc Tuyet PT, Thompson C, Wolbers M, Merson L, et al. An oral preparation of Lactobacillus acidophilus for the treatment of uncomplicated acute watery diarrhoea in Vietnamese children: study protocol for a multicentre, randomised, placebo-controlled trial. Trials. 2013 Jan; 14:27. doi:10.1186/1745-6215-14-27
25. Gaon D, Garmendia C, Murrielo NO, de Cucco Games A, Cerchio A, Quintas R, et al. Effect of Lactobacillus strains (L. casei and L. Acidophillus Strains cerela) on bacterial overgrowth-related chronic diarrhea. Medicina (B Aires). 2002;62(2):159-63.
26. Zhang YC, Zhang LW, Ma W, Yi HX, Yang X, Du M, et al. Screening of probiotic lactobacilli for inhibition of Shigella sonnei and the macromolecules involved in inhibition. Anaerobe. 2012 Oct; 18(5):498-503. doi:10.1016/j.anaerobe.2012.08.007
27. Shornikova AV, Casas IA, Isolauri E, Mykkänen H, Vesikari T. Lactobacillus reuteri as a therapeutic agent in acute diarrhea in young children. J Pediatr Gastroenterol Nutr. 1997 Apr; 24(4):399-404.
28. Callaway TR, Edrington TS, Anderson RC, Harvey RB, Genovese KJ, Kennedy CN, et al. Probiotics, prebiotics and competitive exclusion for prophylaxis against bacterial disease. Anim Health Res Rev. 2008 Dec; 9(2): 217-25. doi:10.1017/S1466252308001540
29. Jain S, Yadav H, Sinha PR, Naito Y, Marotta F. Dahi containing probiotic Lactobacillus acidophilus and Lactobacillus casei has a protective effect against Salmonella enteritidis infection in mice. Int J Immunopathol Pharmacol. 2008 Oct-Dec; 21(4):1021-9.
30. Moorthy G, Murali MR, Devaraj SN. Protective role of lactobacilli in Shigella dysenteriae 1-induced diarrhea in rats. Nutrition. 2007 May;23(5):424-33.
31. Szabo I, Wieler LH, Tedin K, Scharek-Tedin L, Taras D, Hensel A, et al. Influence of a probiotic strain of Enterococcus faecium on Salmonella enterica serovar Typhimurium DT104 infection in a porcine animal infection model. Appl Environ Microbiol. 2009 May; 75(9):2621-8. doi:10.1128/AEM.01515-08
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Soltan Dallal MM, Keshtvarz M, Zamani S, Shirazi L. Evaluation of anti-microbial activity of Lactobacillus acidophillus and Lactobacillus ruteri against entero-pathoges by in vitro and in vivo methods. J Gorgan Univ Med Sci 2016; 18 (1) :45-52
URL: http://goums.ac.ir/journal/article-1-2654-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 1 (3-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.04 seconds with 36 queries by YEKTAWEB 4657