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:: Volume 18, Issue 1 (3-2016) ::
J Gorgan Univ Med Sci 2016, 18(1): 45-52 Back to browse issues page
Evaluation of anti-microbial activity of Lactobacillus acidophillus and Lactobacillus ruteri against entero-pathoges by in vitro and in vivo methods
Soltan Dallal MM * 1, Keshtvarz M2 , Zamani S3 , Shirazi L4
1- Professor, Div. of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , msoltandallal@gmail.com
2- Ph.D Candidate in Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3- Ph.D Candidate in Microbiology, School of Medicine, Tehran University of Medical Sciences. Tehran, Iran
4- M.Sc in Microbiology, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (10999 Views)

Background and Objective: Probiotics are beneficial organisms therapeutic within microbial flora. Shigella, Escherichia coli and Salmonella are the most common cause of intestinal infectious diseases that lead to morbidity and mortality in infant and children worldwide. The aim of this study was to evaluate anti-microbial activity of Lactobacillus acidophillus and Lactobacillus ruteri against entero-pathoges by in vitro and in vivo methods. Methods: In this experimental study, the therapeutic effect of the lactobacillus acidophilus ATCC 4356 and ruteri ATCC 23272 against Shigella sonnei ATCC 9290, Escherichia coli ATCC 25922 and Salmonella enterica BAA-708 were evaluated by in vitro (spot agar) and in vivo (BALB/c mice) methods. Weight improvment and survival rate in mice were recorded. Results: Lactobacillus acidophillus and ruteri had protective and therapeutic effect against diarrhea caused by pathogenic bacteria. Probiotics reduced the weight, colonization of pathogens and increased the survival rate of animals (P<0.05). Conclusion: Lactobacillus acidophillus and ruteri has anti-microbial activity and their consumption can be effective in the prevention and also the treatment of intestinal disease.
Keywords: Entero pathogens, Probiotics, Antibacterial activity, Mouse
Full-Text [PDF 272 kb] [English Abstract]   (19880 Downloads) |   |   Abstract (HTML)  (372 Views)  
Type of Study: Original Articles | Subject: Microbiology
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Soltan Dallal MM, Keshtvarz M, Zamani S, Shirazi L. Evaluation of anti-microbial activity of Lactobacillus acidophillus and Lactobacillus ruteri against entero-pathoges by in vitro and in vivo methods. J Gorgan Univ Med Sci 2016; 18 (1) :45-52
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Volume 18, Issue 1 (3-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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