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Anti-viral effect of methanolic extract of Sea cucumber on HIV-1 virus
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Bahroudi S1   , Nematollahi MA2 , Aghasadeghi MR * 3, Nazemi M4 , Bahroudi M5 , Behrouz B5 |
1- M.Sc in Processing Marine Products, Fisheries Department, Faculty of Natural Resources, University of Tehran, Karaj, Iran
2- Associate Professor, Fisheries Department, Faculty of Natural Resources, University of Tehran, Karaj, Iran
3- Associate Professor, Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, IR Iran , mrasadeghi@pasteur.ac.ir
4- Assistant Professor, Persian Gulf and Oman Sea Ecological Research Institute, Bandar Abbas, Hormozgan, Iran
5- M.Sc in Microbiology, Microbiology Department, Tehran University of Medical Sciences, Tehran, Iran |
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Abstract: (9465 Views) |
| Background and Objective: Sea cucumber (Holothuria leucospilota) is used for food purposes and traditional medicine in the South East and East Asia. This study was done to determine the antiviral effect of methanolic extract, of Holothuria leucospilota species against HIV-1 virus. Methods: In this laboratory study, sea cucumbers were collected from Larak Island, Persian Gulf, Iran at depths of 10-30 m. Methanol solvent was used for extraction process. Extract was concentrated by rotary evaporator at 40-45 degree C, and subsequently was prepared in the form of dry powder using vacuum freeze dryer lyophilization. Results: The extract in 100 and 1000 µg/ml of concentrations inhibited by 94% and 92.5% the replication of HIV-1, respectively. 10 µg/ml of extract had not specific antiviral effect. Approximately the half of concentration of extract (35.89 µg/ml) prevents 50% of proliferation of HIV-1, which was 50% toxic of on host cells (P<0.05). Conclusion: Sea cucumber methanolic body wall extract of Holothuria leucospilota species had no antiviral effect against HIV-1 virus. It can be due to cytotoxic effect of extract on the host cells. |
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| Keywords: Sea cucumber, HIV-1 virus |
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Full-Text [PDF 282 kb]
[English Abstract]
(15504 Downloads)
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Type of Study: Original Articles |
Subject:
Virology
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