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:: Volume 18, Issue 1 (3-2016) ::
J Gorgan Univ Med Sci 2016, 18(1): 114-119 Back to browse issues page
Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells
Fani N1 , Shafiee Ardestani M * 2, Yaghmaei P3 , Assadi A1 , Barzegar Behrouz A4
1- Department of Biochemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Assistant Professor, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran , shafieeardestani@sina.tums.ac.ir
3- Associate Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4- M.Sc in Physiology, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (9753 Views)

Background and Objective: Wilson’s disease (WD) is caused by mutation to the cooer-transporting gene ATP7B. Chelation therapy is the main protochol of treatment for patients with Wilson’s disease. D-penicillamine is one of the well-known chelator agants which is used in WD treatment but it can not enter into the intracellular space.This study was done to evaluate the synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells. Methods: In this descriptive-analytic study, initially 0.01 mm polyethylene glycol (PEG) and 0.0018 mm citric acid, Dendrimer was synthesized. After purification by dialysis bag and lyophilization, 10mg dendrimer was conjugated to 3.3mg D-penicillamine. Nanoconjugated D-penicillamine-dendrimer was injected on Wilson’s model cells. After incubation and centrifugation intracellular measurement of copper concentration and FTIR test were done. Results: Copper accumulation significantly reduced in the HepG2 WD cell by Nanoconjugated D-penicillamine - Dendrimer in compared to D-penicillamine (P<0.05). Copper accumulation was determined to be 46.61. MTT assay showed no toxicological damage in HepG2 WD cell. Conclusion: Nanoconjugated D-penicillamine –Dendrimer can reduces intracellular concentration of Copper.
Keywords: Wilson’s Disease, Nanoconjugated D-penicillamine-dendrimer
Full-Text [PDF 315 kb] [English Abstract]   (18851 Downloads) |   |   Abstract (HTML)  (351 Views)  
Type of Study: Original Articles | Subject: Medical Biotecnology
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Fani N, Shafiee Ardestani M, Yaghmaei P, Assadi A, Barzegar Behrouz A. Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells. J Gorgan Univ Med Sci 2016; 18 (1) :114-119
URL: http://goums.ac.ir/journal/article-1-2585-en.html
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Volume 18, Issue 1 (3-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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