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Association of ApE1 gene Asp148Glu polymorphism and idiopathic male infertility
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Yousefi M1   , Salehi Z *2 , Mashayekhi F3 , Bahadori MH4 |
1- M.Sc student in Cell and Developmental Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
2- Professor, Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran , geneticzs@yahoo.co.uk
3- Professor, Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
4- Associate Professor, Cellular and Molecular Research Center, Faculty of Medical Sciences, Guilan University of Medical Sciences, Rasht, Iran |
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Abstract: (10487 Views) |
| Background and Objective: Despite enormous progress in the understanding of human reproductive physiology, the underlying cause of male infertility remains undefined in about 50.0% of cases, which are referred to as idiopathic infertility and affects about 5.0-7.0% of the general male population. Human apurinic/apyrimidinic endonuclease (ApE1) is a multifunctional protein that has an important role in the base excision repair (BER) pathway. ApE1 SNP T>G found in exon 5 led to substitution of Asp>Glu at codon 148. This study was done to evaluate the association of ApE1 Asp148Glu polymorphism and the risk of idiopathic male infertility. Methods: In this case-control study, blood samples were collected from 90 patients diagnosed with idiopathic male infertility and 90 healthy men, genotyped by Allele-Specific PCR (AS-PCR) method by using specific primers that were designed and the association between genotype and allele frequencies in cases and controls were estimated. Results: There was no significant association between ApE1 gene polymorphism at codon 148 in case and control groups. Conclusion: No significant association was found between the Asp148Glu polymorphism and idiopathic male infertility. |
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| Keywords: Infertility, Male, Polymorphism, ApE1 gene |
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Full-Text [PDF 177 kb]
[English Abstract]
(18218 Downloads)
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Type of Study: Original Articles |
Subject:
Genetic
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