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Effect of Aloe vera gel on TGF-β gene expression in incisional skin wound in BALB/c mice
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Jafarzadeh H1   , Arabi M2 , Najafi N * 3, Ahadi AM4 |
1- M.Sc in Animal Physiology, Department of Biology, Shahrekord University, Shahrekord, Iran
2- Ph.D in Animal Physiology, Department of Biology, Shahrekord University, Shahrekord, Iran
3- M.Sc in Animal Physiology, Department of Biology, Shahrekord University, Shahrekord, Iran , na1365na@gmail.com
4- Ph.D in Genetic, Department of Genetic, Shahrekord University, Shahrekord, Iran |
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Abstract: (11448 Views) |
| Background and Objective: Aloe vera (Aloe barbadensis M.) as a medicinal herb is practiced in wound healing. This study was carried out to assess the effect of Aloe vera gel (mucilage) on TGF-β gene expression in incisional skin wound in BALB/c mice. Method: In this experimental study, 36 BALB/c male mice with weight range 22±2 gr were allocated equally into negative control (no wound), sham-operated (wound treated with physiological serum) and teratment (wound treated with Aloe vera gel). Two equal full-thickness skin wounds of 10±2mm were made on either side of the vertebral column in the sacral region. The animals in the teratment group were received daily, 2 gram of Aloe vera gel (without any bandage) as a thin layer for a period of 16 days. On 8th and 16th post wounding day, TGF-β gene expression in incisional wounds and Malonyldialdehyde (as end-product of lipid peroxidation) in serum samples was measured using RT-PCR and spectrophotometry methods, respectively. Results: TGF-β gene expression in incisional skin wound increased in Aloe vera gel treated group in compared to negative control and sham-operated groups (P<0.05). Malonyldialdehyde concentration was significantly reduced in Aloe vera treated group in comparision with negative control and sham-operated groups. Conclusion: Aloe vera gel can induce growth factor TGF-β gene expression and reducing the lipid peroxidation content can play an important role in incisional skin wound healing process. |
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| Keywords: Skin, Incisional wound, Aloe vera, TGF-β gene, Malonyldialdehyde, Mouse |
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Full-Text [PDF 852 kb]
[English Abstract]
(12343 Downloads)
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Type of Study: Original Articles |
Subject:
Physiology - Pharmacology
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