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Efficacy of ultraviolet radiation on drug susceptibility of Candida Spp. to itraconazole, fluconazole and amphotericin B
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Nowrozi H *   1, Kazemi A2 , Teshfam M3 , Temorian Sh4 , Adimi P5 , Bashashati M6 |
1- Assistant Professor, Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran , nowrozi_h@tums.ac.ir
2- Assistant Professor, Department of Nursing and Midwifery, Islamic Azad University, Varamin - Pishva Branch, Varamin, Iran
3- Assistant Professor, Department of Physiology, Science and Research branch, Islamic Azad University, Tehran, Iran
4- Assistant Professor, Department of Genetics, Faculty of Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
5- Assistant Professor, Department of Medical Mycology, Faculty of Medical Sciences, Islamic Azad University, North Branch, Tehran, Iran
6- Academic Instructor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran |
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Abstract: (24876 Views) |
| Background and Objective: Ultraviolet (UV) radiation is a important disinfectant. Fungal infections with resistant isolates in patients culminate in recurrence of disease even with worse condition. This study was done to evaluate the efficacy of ultraviolet radiation on drug susceptibility of Candida Spp. to itraconazole, fluconazole and amphotericin B. Materials and Methods: This laboratory study was done on 12 Candida spp. isolated from patients according to NCCLS M27- A method. Samples were suspended with sterile saline and optical density was read by spectrophotometer at the wavelength of 530 nm. Serial dilutions (0.0313-16 µg/ml) and (0.0313-128 µg/ml) were supplied for itraconazole, amphotericin and fluconazole, respectively. MICs were determined after 48h incubation at 35°C. Following UV radiation for 1, 2, 5, 10, 60, 90 and 120 seconds MICs were determined, subsequently. Results: The highest MIC pre UV radiation was (>128 µg/ml) for fluconazole. After UV radiation, MICs were steadily decreased for all mentioned drugs while after 10 sec, MICs of itraconazole and amphotericin B were >0.0313 µg/ml. Secondary MICs significantly decreased with respect to MICs obtained in pre UV radiation (P<0.05). Conclusion: UV radiation reduces MICs of Candida spp. to itraconazole, fluconazole, amphotericin B. |
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| Keywords: Candida, Ultraviolet radiation, Itraconazole, Fluconazole, Amphotericin B, Drug resistance |
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Full-Text [PDF 293 kb]
[English Abstract]
(20945 Downloads)
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Type of Study: Original Articles |
Subject:
Mycology
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Nowrozi H, Kazemi A, Teshfam M, Temorian Sh, Adimi P, Bashashati M. Efficacy of ultraviolet radiation on drug susceptibility of Candida Spp. to itraconazole, fluconazole and amphotericin B. J Gorgan Univ Med Sci 2013; 15 (4) :53-58 URL: http://goums.ac.ir/journal/article-1-1870-en.html
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