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Showing 3 results for رحیمی
Enhanced Th1 and Th2 immune response induction by Human Papilloma virus Type 16 E7 DNA vaccine in a tumoric murine model
Alireza Mohebbi, Sanaz Baghban Rahimi, Alijan Tabarraei, Mohsen Saeedi, Mirsaeed Ebrahimzadeh, Leila Alizadeh, Amir Ghaemi,
Volume 4, Issue 2 (10-2016)
Abstract

Background and Objectives: Human papilloma virus (HPV) is known as the etiologic agent of cervical cancer and second common cancer among women. HPV viruses with the elevated risk of infection have more potentiality to cause cancer. The carcinogenesis in these viruses is accomplished by oncoproteins such as E7. Employing DNA vaccines which code specific antigens such as E7 is a novel therapeutic approach against such cancers.

Methods: In the present study, plasmid coding HPV16 E7 was administered intracutaneously to C57BL/6 tumoric mice models for investigation of its immunostimulating potential. PcDNA3.1+ vector was used as control vector. After immunization, spleen of animals were removed. Then, release of lactate dehydrogenase (LDH) was evaluated to address the cytotoxic activity (CTL) induced by cellular immunity in spleenocytes. Interferon-γ (IFN-γ) and interleukin-4 (IL-4) cytokines were also analyzed as profiles of Th1 and Th2, respectively. Anti-inflammatory cytokine interleukin-10 (IL-10) levels were also investigated in tumor microenvironments.

Results: Our results showed that CTL activity was higher among samples receiving HPV16 E7 coding vector in comparison to the group receiving pcDNA3.1+ control vector (P < 0.05). Levels of IFN-γ and IL-4 were also higher in the group receiving HPV16 E7 plasmid in comparison to the control group (P < 0.05). Similarly, IL-10 levels were significantly lower in tumor carrying mice groups receiving HPV16 DNA vaccine compare to PBS and pcDNA3.1 receiving control groups.

Conclusion: HPV16 E7 expressing DNA vaccine could increase the release of LDH due to immune system CTL activity. Elevation in IFN-γ and IL-4 levels as well as IL-10 reduction indicates an increase in both Th1 and Th2 profiles resulted by using potent DNA vaccine coding HPV16 E7 in tumor animal model.


Evaluation of DNA vaccine encoding HPV-16 E7 on Lymphocyte proliferation induction in Human Papilloma Virus-associated tumor animal models
Sanaz Baghbanrahimi, Hoorieh Soleimanjahi, Alireza Mohebbi, Mir Saeid Ebrahimzadeh, Leyla Alizade, Amir Ghaemi,
Volume 5, Issue 1 (5-2017)
Abstract
Background & Objective: Human papillomavirus (HPV) oncoproteins, including E6 and E7 are constitutively expressed in cervical cancer cells. These proteins are ideal targets to be used for developing therapeutic vaccines against existing HPV-associated carcinomas. The aim of this study was to measure the proliferation response rate of splenic lymphocytes derived from E7-HPV16 encoding plasmid injection on the tumor mouse model of papillomavirus.
Method: C57BL/6 mice were inoculated subcutaneous with 5× 10⁵ TC-1 cells in three times with two weeks intervals and then immunized with HPV-16 E7 DNA vaccine. The proliferation response of splenic cells was measured by MTT assay. IL12 cytokine was measured by ELISA assay and the mass of tumor was calculated with caliper for six weeks.
Results: Following the application of DNA vaccines containing E7 therapeutic gene, the proliferative response of splenic cells was provoked significanltly higher than the stimulation in control group (P<0.05). Moreover, the secretion of IL12 was significantly increased in vaccinated mice tumor tissue (P<0.05). The growth of tumor in vaccinated group was markedly decreased in comparison to PBS and pcDNA3 groups (P<0.05).
Conclusion: Our findings revealed that the application of DNA vaccine containing E7 gene in a tumor mouse model may induce anti-tumor cellular immune responses.

Epidemiological Study of the Suburban Accident Mortalities Recorded in Golestan, Iran in 2015
Farzaneh Afkhaminia, Dr Jamshid Yazdani Charati, Elaheh Rahimi, Dr Nourodin Mousavi Nasab,
Volume 6, Issue 1 (3-2018)
Abstract
Background and objectives: Road accidents are one of the most important causes of mortality and severe physical and psychological damage which may lead to adverse social, cultural and economic consequences in the human community. Frequency and severity of road accidents in developing countries are noticeably higher in comparison to developed countries. In Iran, 25% of casualties are due to the abnormal deaths caused by road accidents. It is estimated that more than 22,000 people die due to road accidents every year. The present study aimed to epidemiologically investigate the mortality rate of suburban accidents in Golestan province, Iran.
Methods: This cross-sectional study was conducted using a descriptive approach. Required data were obtained from the traffic police of Golestan province. In total, 2,922 cases of road accidents were investigated in Golestan province in 2015. The analyzed data included the demographic characteristics of the deceased and the environmental and geographical conditions of the accident. Data analysis was performed in SPSS version 20.
Results: Among 2,922 road accidents in Golestan province, 251 cases led to the death of 317 individuals. Most of the accidents leading to death were by automobiles (69.7%) and due to distraction from the road (33.1%), which occurred on main roads (47.8%). Moreover, 29.7% of guilty drivers had not fastened seatbelts. Motorcycle riders and car passengers accounted for the highest percentage of accident victims (30.6% and 25.5%, respectively).
Conclusion: Education and emphasis on the use of seatbelts and motorcycle helmets while driving seem essential to reducing the injuries caused by road traffic accidents. Considering that most road accidents occur due to the distraction of the driver from the road, changing traffic behaviors to improve discipline is of paramount importance.
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