Jorjani Biomedicine Journal

Study the Molecular Association between a Deletion Mutation in CHEK2 gene (5395 bp) and Breast Cancer

Mohammad Shekari

Background & Objectives: Breast cancer is the most common cancer among women and the second most common cause of cancer death. Genetic factors play an important role in the development of breast cancer. Among these genetic factors, CHEk2 (checkpoint kinase 2) gene, as a tumor suppressor gene, plays a critical role in DNA repair. Germline mutations in CEHK2 result in the loss of this feature. One of the mutations in CHEK2 gene is a 5395 bp deletion mutation which has been associated with the increasing risk of Breast Cancer in some populations in the world. In the present study, we investigated the association between a 5395 bp deletion mutation in CHEK2 gene and the risk of Breast Cancer in the women of an Iranian population.

Methods: Pathologic information of 38 cases under the age of 45 and 62 cases over the age of 45 referring to surgery ward of Milad Hospital in Tehran were extracted. 100 healthy controls were included in the study as well. After obtaining informed consent, 5 mL whole blood was taken DNA was successfully isolated. Multiplex PCR was used to investigate the association between a 5395bp deletion mutation in CHEK2 gene and increasing risk of Breast Cancer among patients.

Results: The 5395bp deletion mutation in CHEK2 gene was not found in any of the participating groups of patients or heathy controls.

Conclusion: The present study revealed that there is no significant relation between increasing the risk of Breast Cancer and bearing large deletion mutation in exon 9 and exon 10 of CHECK2 gene.

Study the Effect of Some Prebiotics on Physicochemical and Sensory Properties of Dietary Orange Juice

Seyed Amir Mohgammad Mortazavian

Background and objectives: Fortification of beverages with new functional ingredients such as prebiotics is one of the recent progresses in the field of juice production. Therefore, the objective of the present study was to evaluate the effects of adding some prebiotics such as Inulin and Tagatose on physicochemical and sensory properties of orange juice.

Methods: The prebiotic compounds (Inulin and Tagatose) along with sucrose were added to orange juice in specific proportions and then pasteurized at 90°C. Orange juice treated samples were kept at 4°C (refrigerator temperature) and 25°C (room temperature) for 3 months. Physicochemical (Brix, acidity, sucrose content and pH) and sensory properties of treated samples were evaluated within time intervals of one month.

Results: The results showed that storage temperature had no significant effect on pH, Brix and acidity of all treatments (P>0.05). On the other hand, total sugar content of all treatments had decreased significantly during storage (P<0.05), as the greatest decrease was observed in Inulin-3/Sucrose-3 treatment keeping at room temperature with 33.7%. It was reported that no change in the transparency treatments was observed (P>0.05). Storage of treatments at room temperature had decreased the acceptance of flavors significantly (P<0.05).

Conclusion: The result of this study showed that Inulin in combination with sucrose and Tagatose could be used to produce salutary juice with desirable sensory properties.

Associations between Sports Participation, Variety of Physical Activity and Cardiorespiratory Fitness in Students of Gorgan University of Agricultural Science and Natural Resources

Samaneh Haji Hosseini

Background and Objectives: With respect to lack of information about the effect of intensity differences, type of sport participation and their relationships with the other health factors such as cardiorespiratory fitness (CRF), the present study was conducted to evaluate the associations between sports participation, levels of Physical Activity (PA) and CRF.

Methods: The present analytical study was conducted on 406 undergraduate students aging 18-21 years from Gorgan University of Agricultural Science and Natural Resources in second semester of 2014. CRF was tested with Rockport Walk Test among all participants. Sports participation was assessed by Ledent questionnaire and habitual physical activity (PA) data was evaluated using metabolic equivalent (METs) self-reported questionnaire. All analyses were carried out using SPSS version 20.0. An independent T-test, Chi-square statistic, ANOVA and regression were employed to analyze data statistically.

Results: The participants with desired CRF and acceptable levels of PA were significantly engaged more active in organized or unorganized sport activities and also competitive sports compared to others (P < 0.05). Individuals with at least 3 hours of moderate to vigorous PA per day had a better CRF (P<0.05).Regression results showed that participation in any level of competitive sports regardless of winning or loss guarantee the CRF to some extent.

Conclusion: Participation in competitive sports along with adopting active life style (2:30 min PA with moderate intensity and 30 min of competitive sport) may increase the chance of gaining CRF.

Relationship between Helicobacter pylori infection with common dietary habits in Khuzestan province in patients with dyspepsia referred to Imam Khomeini Hospital, Ahwaz

Hossein Khadem Haghighian

Background and Objective: Helicobacter pylori (HP) infection is one of the main risk factors for stomach illness. This study aimed to investigate the relationship between Helicobacter pylori infection and common dietary habits in patients with dyspepsia.

Methods: This cross-sectional study was performed on individuals referred for gastrointestinal endoscopy (N: 374) using a one-year food frequency questionnaire and a 24 hour dietary recall for three days. Tissue samples from the antrum, body and fundus were derived and kept in glass containers including formalin buffer for further pathologic examinations. All data were analyzed using SPSS version 13.0. Independent-Sample t test was used to compare the means. P value less than 0.05 was considered significant.

Results: The study population was comprised of 214 healthy subjects (57%) and 160 patients (43%). The average daily intake of sausages (p = 0.001), burgers (p = 0.002) was higher in patients compared to the healthy group significantly. The average daily intake of fish (p = 0.001) and weekly intake of tomato (p = 0.001), onions (p = 0.002) and citrus (p = 0.001) was lower among patients in comparison to healthy subjects significantly.

Conclusion: The high consumption of fruits and vegetables and low intake of red meat were of dietary habits among healthy individuals. Although, high consumption of processed meat was of dietary habits among patients infected with H. pylori. Therefore, a proper diet can be recommended as a public health policy to the general public.

The Relationship between Nursing Care Quality and Patients Satisfaction among Hospitals affiliated to Zahedan University of Medical Sciences in 2014

Afsaneh Ghasemi

Background and Objective: Obtaining the means of promoting nursing care quality have been always considered by nursing managers. The present study was conducted to determine the relationship between nursing care quality and patients satisfaction in the hospitals affiliated to Zahedan University of Medical Sciences in 2014.

Methods: The present cross-sectional study was accomplished on 68 employed nurses and 204 patients during a 4 month random sampling time interval. Three patients per nurse who had utilized at least 72 hours of nursing cares and were about to discharge at the time of research were chosen. Data collection tolls were the standardized questionnaires including nursing care quality measurement tool (Quality patient care scale: QUALPAC) and Patient Satisfaction Instrument (PSI). The data were analyzed using Chi-square, One-Way ANOVA and Pearson correlation coefficient tests.

Results: The average age of the nurses and patients were respectively 30.8±4.9 and 37.9±10.7. The average score for QUALPAC was 61.3±12.5 and the quality of care was desirable in 55% of cases. Moreover, the average score for the satisfaction of patients was 71.8±10.9 and the patients expressed an intermediate satisfaction in 61 percent of cases. There was a positive significant correlation between the nursing care and patient satisfaction (P=0001).

Conclusion: It seems as if the health care system can provide the satisfaction of patients by enhancing nursing care quality or to employing more skilled nurses.

The Prevalence and the Affecting Factors of Obesity in Women of Kermanshah

Mitra Darbandi

Background & Objective: Obesity is a public health problem in modern societies which is more prevalent among women compared to men. This study was conducted to aim the prevalence of obesity and the related factors in women of Kermanshah.

Materials and Methods: In the present cross-sectional study, 687 women aging 25-65 years were enrolled using multi staged cluster sampling method from 6 regions of Kermanshah city. Data collection tools were a demographic questionnaire, Food Frequency (FFQ) and physical activity questionnaires. General Obesity was defined as BMI≥30 kg/m2 and abdominal obesity was defined as waste hip ratio (WHR) over 0.85cm. All data were analyzed using correlation coefficient, Logistic regression-test and x² by SPSS software.

Results: Overweight and obesity was observed in 39.4% and 21.9 % of women respectively. 57.5% of studied subjects suffered from abdominal obesity. The odds ratios for obesity among illiterate (OR=1.882, P=0.05), low socioeconomic status (OR=1.867, P=0.002), and having more than four pregnancies (OR=1.875, P=0.01) were higher than other women. Odds ratio of fatty liver (OR: 3.818) and diabetes (OR: 3.077) was higher among obese subjects than normal individuals. (P=0.02).There was a positive correlation between obesity and marital status, number of pregnancies, family population size and unhealthy dietary habits (P<0.001). Moreover, there was a negative relationship between obesity and education level, employment and high socioeconomic status (P<0.001).

Conclusions: Regarding the side effects of obesity and overweight, lifestyle modification and increasing nutritional knowledge among women using appropriate methods is highly recommended.

Correction the Bias of Odds Ratio resulting from the Misclassification of Exposures in the Study of Environmental Risk Factors of Lung Cancer using Bayesian Methods

Bagher pahlavanzade

Background & Objective: Inability to measure exact exposure in epidemiological studies is a common problem in many studies, especially cross-sectional studies. Depending on the extent of misclassification, results may be affected. Existing methods for solving this problem require a lot of time and money and it is not practical for some of the exposures. Recently, new methods have been proposed in 1:1 matched case–control studies that have solved these problems to some extent. In the present study we have aimed to extend the existing Bayesian method to adjust for misclassification in matched case–control Studies with 1:2 matching.

Methods: Here, the standard Dirichlet prior distribution for a multinomial model was extended to allow the data of exposure–disease (OR) parameter to be imported into the model excluding other parameters. Information that exist in literature about association between exposure and disease were used as prior information about OR. In order to correct the misclassification Sensitivity Analysis was accomplished and the results were obtained under three Bayesian Methods.

Results: The results of naïve Bayesian model were similar to the classic model. The second Bayesian model by employing prior information about the OR, was heavily affected by these information.

The third proposed model provides maximum bias adjustment for the risk of heavy metals, smoking and drug abuse. This model showed that heavy metals are not an important risk factor although raw model (logistic regression Classic) detected this exposure as an influencing factor on the incidence of lung cancer. Sensitivity analysis showed that third model is robust regarding to different levels of Sensitivity and Specificity.

Conclusion: The present study showed that although in most of exposures the results of the second and third model were similar but the proposed model would be able to correct the misclassification to some extent.

Study the Relationship between Internet-related Lifestyle and Loneliness and Social Support among Internet Users in Ilam University of Medical Sciences

Zahra Shafieian

Background & Objective: Using internet have had a significant impact on the lifestyle changes of internet clients which can affect their health. The aim of this study was to survey the relationship between lifestyle related to internet with loneliness and social support of the internet clients in Ilam University of medical sciences.

Methods: This study was a cross sectional study which was performed in 2014 on 400 university students and personnel of Ilam University of medical sciences using stratified random sampling method.Data collection instrument was a questionnaire comprising of four sections: demographic information, lifestyle related to internet questionnaire , loneliness and social supports questionnaires.All data were analyzed using SPSS software by Mann Withney and Kruscall- Wallis tests and linear regression test.

Results: The linear regression results showed that there was a significant relationship between loneliness and lifestyle related to the internet, gender, marital status, occupational statues and age (P<0.01). There was also a significant relationship between social support and lifestyle related to the internet and age (P<0/05). The results showed that there was a significant relationship between loneliness and social support with marital status, educational degree and internet usage (P<0/01).

Conclusion: According to the significant relation between loneliness and social support with the lifestyle related to the internet, and regarding the inevitability of Internet, it seems more supporting the students and implementing the educational programs for university clients about suitable using of internet is necessary.

The Relationship between Intellectual Intelligence and Emotional Intelligence and some Demographic variables among Students of the Faculty of Nursing and Midwifery, Ilam University of Medical Sciences in 2014

Hamed Tavan

Background and Objective: There is a relationship between emotional intelligence and spiritual intelligence. Therefore, this study was aimed to investigate the relationship between intellectual intelligence and emotional intelligence and some demographic variables among students of Nursing and Midwifery Faculty, Ilam University of Medical Sciences.

Methods: Using a cross-correlation method of study, the standard 24-item questionnaire for spiritual intelligence and the standard 90-item questionnaire for emotional intelligence was subjected to 118 university students of Nursing and Midwifery faculty by simple random sampling. At the beginning of the questionnaire, demographic information were derived from the students. All data were analyzed by SPSS software and using Independent t-test, One-way ANOVA and Pearson Correlation.

Results: The samples were comprised of 56% female and 46% male and the average score of spiritual intelligence among students was 68.5 while the average score of emotional intelligence was 305. The results showed that there was a significant relationship between emotional intelligence, spiritual intelligence and gender while there was no relationship with the field of study.

Conclusion: The spiritual intelligence and emotional intelligence was higher among women compared to men, and there was a significant relationship between emotional intelligence and spiritual intelligence so that boosting emotional intelligence can improve the emotional intelligence.