Jorjani Biomedicine Journal

en Investigating the impact of aerobic training on myokine gene expression in the skeletal muscle of wistar rats بهداشت Health تحقیقی Original article <pre style="text-align:justify"> Background: Skeletal muscle is a tissue that secretes myokines from muscle cells in response to training stimuli and muscle contractions. Therefore, this study aimed to investigate the effect of 4-week moderate-intensity aerobic exercise on the expression of three genes: apelin, decorin, and musclin in the skeletal muscle fibers of Wistar rats. In addition, the study examined the changes in gene expression levels during the training period. Methods: The present study enrolled 16 male Wistar rats with an approximate age of eight weeks and a weight range of 200-220 grams. They were randomly divided into two equal groups: An aerobic exercise group (n=8) and a control group (n=8). During the four-week training period, the experimental group performed aerobic exercises on a treadmill with an intensity of 50-70% of their maximum power, for three sessions per week, while the control group did not engage in any activity. After completing the training period, the rats were sacrificed 48 hours later to evaluate the gene expression of the study variables using the real-time PCR method for tissue analysis. An independent t-test was used to examine the difference between groups, and statistical significance was set at P <0.05. Results: The independent t-test results indicated that the gene expression levels of all three variables: Musclin, decorin, and apelin, were significantly higher in the aerobic exercise group compared to the control group. Conclusion: The study findings suggest that aerobic exercise can potentially to increase the gene expression of three specific myokines:  apelin, decorin, and musclin in skeletal muscles. These myokines are known to play an essential role in energy homeostasis, and their increased expression levels could have potential health benefits for individuals engaged in aerobic exercise. </pre> Moderate intensity aerobic exercise,Apelin,Decorin,Musclin protein,Energy homeostasis associated protein 20 24 http://goums.ac.ir/jorjanijournal/browse.php?a_code=A-10-924-1&slc_lang=en&sid=1 Seyed Rafie Shafabakhsh Shafabakhsh.s1@cfu.ac.ir 100319475328460010767 0000000215071982 No Department of Sports Physiology, Faculty of Physical Education, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran yaser kazemzadeh Yaser.kazemzadeh@yahoo.com 100319475328460010768 0000000324608363 Yes Department of Sports Physiology, Faculty of Physical Education, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran Hossein Shirvani Shirvani.h2006@gmail.com 100319475328460010769 000000020696958X No Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Sanaz Mirzaiyan Shanjani san_mir2000@yahoo.com 100319475328460010770 0000000248344975 No Department of Sports Physiology, Faculty of Physical Education, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran Saeed Sedaghati sa49sd@gmail.com 100319475328460010771 0000000268021460 No Department of Sports Management, Faculty of Physical Education, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran