en علوم مولکولی Molecular Sciences تحقیقی Original article <div style="text-align: justify;"><span style="line-height:2;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong><i>Background and <span style="color:black">Objectives:</span></i></strong></span></span> <span style="font-family:Times New Roman;"><span style="font-size:14px;"><span style="color:black">The inability of classic fluorescence-activated cell sorting to single cancer cell sorting is one of the most significant drawbacks of this method. The sorting of cancer cells in microdroplets significantly influences our ability to analyze cancer cell proteins. <b><i></i></b></span><br> <b><i><span style="color:black">Material and Methods:</span></i></b> <span lang="EN-GB"><span style="color:black">We</span></span><span style="color:black"> adapted a developed microfluidic device as a 3D in vitro model to sorted MCF-7 cancer cells on a chip.</span> <span style="color:black">A prefabricated microfluidic droplet chip was used in this research. Then, with the help of synthesized fluorescent probes, MCF-7 cancer cells were separated from normal cells.</span><b><i><span dir="RTL" lang="FA"><span style="color:black"></span></span></i></b><br> <b><i><span style="color:black">Results: </span></i></b><span style="color:black">This research presents a modification of GQD bead for high-throughput analysis and sorting single cancer cells. We elaborate a binding assay as an example of this approach for detecting MCF-7 cancer cell lines. Graphene quantum dot-decorated mesoporous silica nanoparticles (GQD@MSNPs) act as fluorescent optical beads coated in microfluidic droplets. The fluorescent beads capture cancer cells. To enable droplet sorting at 200 Hz and cell enrichment, a measurable fluorescence signal is generated when cancer cells bind to these beads and boost the drop&#39;s fluorescence emission. <b><i></i></b></span><br> <b><i><span style="color:black">Conclusion:</span></i></b> Herein, we report in vitro results showing that the as-prepared GQD<span style="color:black">@</span>MSNs have exceptional luminous characteristics. The specific surface area and pore volume of GQD-MSNs were found to be 50% and 40% higher than those of pure MSNs, which is rather remarkable. Because of these improved qualities, GQD<span style="color:black">@</span>MSNs are demonstrated a large sorting capacity that makes them ideal for diagnosis.</span></span></span><span style="font-family:Times New Roman;"><span style="font-size:14px;"><span style="line-height:115%"><span style="line-height:115%"><span style="color:black"></span></span></span></span></span></div> Microfluidics,Quantum Dots,Fluorescent Antibody Technique, 43 50 http://goums.ac.ir/jorjanijournal/browse.php?a_code=A-10-905-1&slc_lang=en&sid=1 Arash Ramedani arashramedani8@gmail.com 10031947532846008346 10031947532846008346 No Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran Abdolreza Simchi simchi@sharif.edu 10031947532846008347 10031947532846008347 Yes Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran/ Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran Omid Sabzevari omid@tums.ac.ir 10031947532846008348 10031947532846008348 No Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, Iran/ Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran