en پزشکی عمومى General medicine تحقیقی Original article <div style="text-align: justify;"><strong>Background and objectives</strong>: Proper nutrition and exercise are two effective factors in improving liver function in old age. The aim of this study was to investigate the effect of aerobic training (T) with consumption of Citrus aurantium (CA) on SIR1 and PGC-1&alpha; gene expression levels in the liver tissue of elderly rats.<span dir="RTL"> </span><br> <strong>Method</strong>: In this experimental study, 25 elderly female rats were placed in five groups of 5 rats, including 1) control, 2) sham, 3) CA, 4) T and 5) T+CA. Over the course of eight weeks, groups 4 and 5 ran on the treadmill three sessions per week at 65 to 75 percent of the maximum running speed, and groups 3 and 5 received 300 mg/kg/day of CA extract peritoneally. Forty- eight hours after the last training session and CA consumption, SIR1 and PGC-1&alpha; gene expression levels in the liver tissue were measured by real-time PCR method. In order to analyze the findings, the Shapiro-Wilk, one-way ANOVA with Tukey&rsquo;s <em>post- hoc </em>tests were used (P&le;0.05).<br> <strong>Results</strong>: T had a significant effect on increasing SIR1 (P = 0.009) and PGC-1&alpha; (P = 0.001) gene expression levels; CA had a significant effect on reducing SIR1 gene expression levels and increasing PGC-1&alpha; (P = 0.001); T + CA had a greater effect on increasing PGC-1&alpha; gene expression levels than T and CA (P = 0.001).<br> <strong>Conclusion</strong>: Although eight weeks of T and CA consumption alone appear to improve PGC-1&alpha; gene expression levels in the liver tissue of elderly rats, concurrent T and CA consumption has more favorable effects than each of them alone.<span dir="RTL"></span></div> Training,Citrus aurantium,Liver,Metabolism,Elderly, 57 65 http://goums.ac.ir/jorjanijournal/browse.php?a_code=A-10-697-4&slc_lang=en&sid=1 Zinab Shykholeslami z.shykholeslami@gmail.com 10031947532846005506 10031947532846005506 No Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Ahmad Abdi a.abdi58@gmail.com 10031947532846005507 10031947532846005507 Yes Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Alireza Barari alireza54.barari@gmail.com 10031947532846005508 10031947532846005508 No Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Seyed Ali Hosseini alihoseini_57@miau.ac.ir 10031947532846005509 10031947532846005509 No Department of Sport Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran